Singh, Jay and Khanra, Partha and Kuila, Tapas and Srivastava, Manish and Das, Ashok K. and Kim, Nam Hoon and Jung, Bong Joo and Kim, Da Yeong and Lee, Seung Hee and Lee, Dong Won and Kim, Dae-Ghon and Lee, Joong Hee (2013) Preparation of sulfonated poly(ether–ether–ketone) functionalized ternary graphene/AuNPs/chitosan nanocomposite for efficient glucose biosensor. Process Biochemistry, 48 (11). pp. 1724-1735.

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A facile method of preparing water-dispersible sulfonated graphene (SPG) using sulfonated poly(ether–ether–ketone) organic polymer as a modifier was realized. A glucose biosensor was fabricated by immobilizing glucose oxidase (GOx) on the surface of AuNPs used to modify SPG and chitosan (CH) deposited on an indium tin-oxide (ITO) glass electrode by a solution casting method. Morphological and structural characterizations confirm that the AuNPs can be efficiently applied to the SPG–CH matrix. The amperometric response of the GOx/SPG–AuNPs–CH/ITO bioelectrode shows a broad linear range of 0.5 to 22.2 mM, with a limit of detection of 0.13 mM and a high sensitivity of 6.51 μA/(mM cm2). The excellent performance of the constructed biosensor is attributed to the large surface-to-volume ratio and electron transfer ability of SPG, the high catalytic activity of the AuNPs, and the good biocompatibility of CH. In addition, the sensor has important advantages, such as its simple preparation, fast response time (10 s), good stability (70 days), and high reproducibility. Favorable results upon examining the electrochemical response for the determination of glucose in human blood serum were obtained, without the assistance of a negligible effect of interfering bio-analytes. The results of studies show that the ternary SPG–AuNPs–CH nanocomposite may offer a new approach for developing novel types of highly sensitive and stable electrochemical biosensors.

Item Type: Article
Subjects: Sensor
Depositing User: Dr. Sarita Ghosh
Date Deposited: 21 Sep 2016 12:03
Last Modified: 21 Sep 2016 12:03

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